Effects of perhexiline-induced fuel switch on the cardiac proteome and metabolome.

Yin, Xiaoke, Dwyer, Joseph, Langley, Sarah R. ORCID: https://orcid.org/0000-0003-4419-476X, Mayr, Ursula, Xing, Qiuru, Drozdov, Ignat, Nabeebaccus, Adam, Shah, Ajay M., Madhu, Basetti, Griffiths, John, Edwards, Lindsay M. and Mayr, Manuel 2013. Effects of perhexiline-induced fuel switch on the cardiac proteome and metabolome. Journal of Molecular and Cellular Cardiology 55 , pp. 27-30. 10.1016/j.yjmcc.2012.12.014

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Abstract

Perhexiline is a potent anti-anginal drug used for treatment of refractory angina and other forms of heart disease. It provides an oxygen sparing effect in the myocardium by creating a switch from fatty acid to glucose metabolism through partial inhibition of carnitine palmitoyltransferase 1 and 2. However, the precise molecular mechanisms underlying the cardioprotective effects elicited by perhexiline are not fully understood. The present study employed a combined proteomics, metabolomics and computational approach to characterise changes in murine hearts upon treatment with perhexiline. According to results based on difference in-gel electrophoresis, the most profound change in the cardiac proteome related to the activation of the pyruvate dehydrogenase complex. Metabolomic analysis by high-resolution nuclear magnetic resonance spectroscopy showed lower levels of total creatine and taurine in hearts of perhexiline-treated mice. Creatine and taurine levels were also significantly correlated in a cross-correlation analysis of all metabolites. Computational modelling suggested that far from inducing a simple shift from fatty acid to glucose oxidation, perhexiline may cause complex rebalancing of carbon and nucleotide phosphate fluxes, fuelled by increased lactate and amino acid uptake, to increase metabolic flexibility and to maintain cardiac output. This article is part of a Special Issue entitled "Focus on Cardiac Metabolism".

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Elsevier
ISSN:	0022-2828
Date of First Compliant Deposit:	24 October 2023
Date of Acceptance:	14 December 2012
Last Modified:	24 Oct 2023 14:08
URI:	https://orca.cardiff.ac.uk/id/eprint/162162

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