X-ray scattering imaging of intervertebral discs under load

Bell, James ORCID: https://orcid.org/0000-0001-8371-9851, Owen, David, Meek, Keith ORCID: https://orcid.org/0000-0002-9948-7538, Terrill, Nick, Sanchez-Weatherby, Juan and Maitre, Christine Le 2022. X-ray scattering imaging of intervertebral discs under load. Presented at: The Society for Back Pain Research (SBPR) Annual General Meeting, Coventry, 30 June 2022. Orthopaedic Proceedings. , vol.104-B (Supp 9) p. 26. 10.1302/1358-992X.2022.9.026

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Abstract

Background An improved understanding of intervertebral disc (IVD) structure and function is required for treatment development. Loading induces micro-fractures at the interface between the nucleus pulposus (NP) and the annulus fibrosus (AF), which is hypothesized to induce a cascade of cellular changes leading to degeneration. However, there is limited understanding of the structural relationship between the NP and AF at this interface and particularly response to load. Here, X-ray scattering is utilised to provide hierarchical morphometric information of collagen structure across the IVD, especially the interface region under load. Methodology IVDs were imaged using the I22 SAXS/WAXS beamline at Diamond Light Source. Peaks associated with the D-banded structure of collagen fibrils were fitted to quantify their azimuthal distribution, as well the magnitude and direction of internal strains under static and applied strain (0–20%). Results IVD tissue regions exhibited structural “AF-like” and “NP-like” fingerprints. Demonstrating high internal strains on collagen fibres particularly within the NP region of the disc. AF and NP regions showed distinct collagen orientation and internal strains with an apparent lack of bracing structure seen at the interface between the differential mechanical tissues. X-ray scattering under tensile strain provided structural information at high resolution, with clear differences observed between normal and degenerate discs under load. Conclusion X ray scattering has been utilised to develop an improved understanding of collagen structure across the intervertebral disc which can be utilised to gain an increased understanding of load induced propagation of micro fissures and disc degeneration.

Item Type:	Conference or Workshop Item (Other)
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences
Additional Information:	Abstract
Last Modified:	09 Oct 2023 09:45
URI:	https://orca.cardiff.ac.uk/id/eprint/162650

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