Two novel PCR-based assays for sexing of Silene latifolia and Silene dioica plants

Hewett, Anna M., Sanchez Vilas, Julia ORCID: https://orcid.org/0000-0002-4049-8443 and Hailer, Frank ORCID: https://orcid.org/0000-0002-2340-1726 2024. Two novel PCR-based assays for sexing of Silene latifolia and Silene dioica plants. MethodsX 12 , 102708. 10.1016/j.mex.2024.102708

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Abstract

Silene latifolia and S. dioica are model systems in studies of plant reproduction, chromosome evolution and sexual dimorphism, but sexing of plants based on morphology is only possible from flowering stage onwards. Both species show homogametic females (XX) and heterogametic males (XY). • Here we developed two assays (primer pairs ss816 and ss441) for molecular sexing of S. latifolia and S. dioica, targeting length polymorphisms between the X and Y-linked copies of the spermidine synthase gene. The two assays were successful in identifying known (flowering-stage) males and females from UK and Spanish populations, with an error rate of 3.1% (ss816; successful for both species) and 0% (ss441, only successful for S. latifolia). Our assays therefore represent novel tools for rapid, robust and simple determination of the genotypic sex of S. latifolia and S. dioica.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Elsevier
ISSN:	2215-0161
Date of First Compliant Deposit:	10 April 2024
Date of Acceptance:	9 April 2024
Last Modified:	22 Apr 2024 15:01
URI:	https://orca.cardiff.ac.uk/id/eprint/167869

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