UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium

Palma, Leopoldo, Ruiz de Escudero, Inigo, Maneru-Oria, Francisco, Berry, Colin ORCID: https://orcid.org/0000-0002-9943-548X and Caballero, Primitivo 2024. UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium. Toxicon: An Interdisciplinary Journal on the Toxins Derived from Animals, Plants and Microorganisms 247 , 107807. 10.1016/j.toxicon.2024.107807

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Abstract

In this study, secretable Vip3Ag4 protein was encapsulated in Bacillus megaterium and used for quantitative bioassays, in order to determine the UV photoprotective capacity of the cell, for preventing inactivation of the insecticidal activity of the protein. The non-encapsulated and purified protein was exposed to the UV light showing a LC50 of 518 ng/cm2 against Spodoptera littoralis larvae, whereas the exposed encapsulated protein exhibited 479 ng/cm2. In addition to the capability to accumulate Vip3 proteins for the development of novel insecticidal formulates, the B. megaterium cell has demonstrated to provide moderate protection against the deleterious action of UV light.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Elsevier
ISSN:	0041-0101
Date of First Compliant Deposit:	9 July 2024
Date of Acceptance:	12 June 2024
Last Modified:	09 Jul 2024 10:15
URI:	https://orca.cardiff.ac.uk/id/eprint/169809

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