Wired for success: Probing the effect of tissue-engineered neural interface substrates on cell viability

Nascimento do Teixeira Adriana, Alexandre, Mendes, Alexandre X, Duchi, Serena, Duc, Daniela ORCID: https://orcid.org/0000-0002-9011-0873, Aguilar, Lilith C, Quigley, Anita F, Kapsa, Robert M I, Nisbet, David R, Stoddart, Paul R, Silva, Saimon M. and Moulton, Simon E 2024. Wired for success: Probing the effect of tissue-engineered neural interface substrates on cell viability. ACS Biomaterials Science & Engineering 10 (6) , pp. 3775-3791. 10.1021/acsbiomaterials.4c00111 Item availability restricted.

	PDF - Accepted Post-Print Version Restricted to Repository staff only until 9 May 2025 due to copyright restrictions. Download (1MB)
	PDF - Supplemental Material Restricted to Repository staff only until 9 May 2025 due to copyright restrictions. Download (491kB)

Abstract

This study investigates the electrochemical behavior of GelMA-based hydrogels and their interactions with PC12 neural cells under electrical stimulation in the presence of conducting substrates. Focusing on indium tin oxide (ITO), platinum, and gold mylar substrates supporting conductive scaffolds composed of hydrogel, graphene oxide, and gold nanorods, we explored how the substrate materials affect scaffold conductivity and cell viability. We examined the impact of an optimized electrical stimulation protocol on the PC12 cell viability. According to our findings, substrate selection significantly influences conductive hydrogel behavior, affecting cell viability and proliferation as a result. In particular, the ITO substrates were found to provide the best support for cell viability with an average of at least three times higher metabolic activity compared to platinum and gold mylar substrates over a 7 day stimulation period. The study offers new insights into substrate selection as a platform for neural cell stimulation and underscores the critical role of substrate materials in optimizing the efficacy of neural interfaces for biomedical applications. In addition to extending existing work, this study provides a robust platform for future explorations aimed at tailoring the full potential of tissue-engineered neural interfaces.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Healthcare Sciences Pharmacy
Publisher:	American Chemical Society
ISSN:	2373-9878
Date of First Compliant Deposit:	7 October 2024
Date of Acceptance:	29 April 2024
Last Modified:	07 Nov 2024 07:15
URI:	https://orca.cardiff.ac.uk/id/eprint/169896

Actions (repository staff only)

Edit Item