Zheng, Keke, Zhong, Jingxiao, Hu, Jingrui, Nebbiolo, Eve, Sanchez-Weatherby, Juan, Tang, Tengteng, Landis, William J., Chen, Junning, Winlove, Peter, Sherlock, Benjamin E. and Bell, James  ORCID: https://orcid.org/0000-0001-8371-9851
2024.
Effects of mineralization on thehierarchical organization of collagen—asynchrotron X-ray scattering and polarizedsecond harmonic generation study.
Interface Focus
14
, 20230046.
10.1098/rsfs.2023.0046
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Abstract
The process of mineralization fundamentally alters collagenous tissuebiomechanics. While the structure and organization of mineral particleshave been widely studied, the impact of mineralization on collagen matrixstructure, particularly at the molecular scale, requires further investigation.In this study, synchrotron X-ray scattering (XRD) and polarization-resolvedsecond harmonic generation microscopy (pSHG) were used to studynormally mineralizing turkey leg tendon in tissue zones representingdifferent stages of mineralization. XRD data demonstrated statisticallysignificant differences in collagen D-period, intermolecular spacing, fibriland molecular dispersion and relative supramolecular twists betweennon-mineralizing, early mineralizing and late mineralizing zones. pSHGanalysis of the same tendon zones showed the degree of collagen fibrilorganization was significantly greater in early and late mineralizingzones compared to non-mineralizing zones. The combination of XRDand pSHG data provide new insights into hierarchical collagen–mineralinteractions, notably concerning possible cleavage of intra- or interfibrillarbonds, occlusion and reorganization of collagen by mineral with time. Thecomplementary application of XRD and fast, label-free and non-destructivepSHG optical measurements presents a pathway for future investigationsinto the dynamics of molecular scale changes in collagen in the presence ofincreasing mineral deposition.
| Item Type: | Article |
|---|---|
| Date Type: | Published Online |
| Status: | Published |
| Schools: | Optometry and Vision Sciences |
| Publisher: | Royal Society, The |
| ISSN: | 2042-8898 |
| Funders: | EPSRC, MRC, ImagingBioPro |
| Date of First Compliant Deposit: | 26 September 2024 |
| Date of Acceptance: | 22 April 2024 |
| Last Modified: | 22 Oct 2024 08:30 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/172407 |
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