Bespoke Human Hypertrophic Chondrocytic Cell Lines Provide the Osteoinductive Signals Required for Vascularized Bone Formation

Stringer, Bradley, Waddington, Rachel J. ORCID: https://orcid.org/0000-0001-5878-1434, Sloan, Alastair James ORCID: https://orcid.org/0000-0002-1791-0903, Phillips, Ian, Telford, Gary, Hughes, David, Craig, Geoffrey, Gangemi, Lavinia, Brook, Ian, Freeman, Christine, Cao, Xuesong, Gosal, Mandeep, Smith, Simon, Russell, Graham and Foster, George A. 2007. Bespoke Human Hypertrophic Chondrocytic Cell Lines Provide the Osteoinductive Signals Required for Vascularized Bone Formation. Tissue Engineering 13 (1) , pp. 133-145. 10.1089/ten.2006.0111

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Abstract

Hypertrophic cartilage provides themorphological and biochemical template for orchestrating bone growth. To produce a bone-inductive material such as hypertrophic cartilage for clinical use, we have conditionally immortalized hypertrophic chondrocytic cells fromhuman femur and expanded them in vitro through more than 145 divisions. The clonal cell lines generated by this process consistently express signals that induce both rat and human marrow cells to differentiate in vitro into osteoblastic cells. Further, implantation of the cellfree extracellular matrix from the immortalized chondrocytic cells causes vascularized bone to form in vivo in bony defects, but not in ectopic sites such as skeletal muscle. This study shows that molecular techniques can be used to generate bespoke human cell lines for bone tissue engineering. It also demonstrates thatmatrix material generated from human immortalized hypertrophic chondrocytic cells may provide an abundant, efficacious, and safer alternative to bone autograft—the currently preferred material for fracture repair.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Dentistry
Subjects:	Q Science > QH Natural history > QH301 Biology R Medicine > RK Dentistry
Publisher:	Mary Ann Liebert
ISSN:	1076-3279
Last Modified:	18 Oct 2022 14:28
URI:	https://orca.cardiff.ac.uk/id/eprint/17660

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