Differentiation of human embryonic/induced-pluripotent stem cells to retinal ganglion cells.

Esmaeili, Maryam and Mead, Ben ORCID: https://orcid.org/0000-0001-5855-0097 2024. Differentiation of human embryonic/induced-pluripotent stem cells to retinal ganglion cells. Mead, Ben, ed. Retinal Ganglion Cells: Methods and Proctocols, Vol. 2708. Methods in Molecular Biology, New York, NY: Humana Press, Springer, pp. 41-48. (10.1007/978-1-0716-3409-7_5)

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Abstract

The generation of retinal ganglion cells (RGCs) differentiated from human embryonic stem cell (hESC) or induced-pluripotent stem cells (iPSC) could aid with understanding of human RGC development, neuronal biology, drug discovery, potential cell-based therapies, and gene regulation. Here, we present a protocol for differentiation of hESC to RGCs using a 40-day protocol, significantly shorter than typical retinal organoids while still yielding cells with RGC-enriched markers and show physiological and morphological properties typical of RGCs.

Item Type:	Book Section
Date Type:	Publication
Status:	Published
Schools:	Schools > Optometry and Vision Sciences
Publisher:	Humana Press, Springer
ISBN:	9781071634097
Last Modified:	04 Jul 2025 13:15
URI:	https://orca.cardiff.ac.uk/id/eprint/179501

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