Protocol for quantifying mitotic progression in adherent and suspension cells by live-cell imaging

Kamranvar, Siamak A. 2026. Protocol for quantifying mitotic progression in adherent and suspension cells by live-cell imaging. STAR Protocols 7 (4) , 104899. 10.1016/j.xpro.2026.104899

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Abstract

Live-cell imaging is used to monitor mitotic progression in adherent cells; however, its application to suspension cells remains limited. Here, we present a protocol for quantifying mitotic progression in adherent and suspension cells by monitoring chromatin condensation using the live-cell DNA probe SPY- DNA. We describe steps for cell synchronization, cell plating, SPY-DNA labeling, and live-cell imaging. We then detail procedures for surface plot visualization and intensity profile analysis. This approach enables direct comparison of mitotic pro gression between adherent and suspension cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Medicine
Publisher:	Cell Press
ISSN:	2666-1667
Date of First Compliant Deposit:	22 October 2026
Last Modified:	22 Oct 2026 10:00
URI:	https://orca.cardiff.ac.uk/id/eprint/190367

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