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PCR primers for microsatellite loci in a Madagascan waterbird, the Sakalava Rail (Amaurornis olivieri)

Brede, Edward G., Long, Peter, Zefania, Sama, Rabenandrasana, Marc, Székely, Tamás and Bruford, Michael William 2010. PCR primers for microsatellite loci in a Madagascan waterbird, the Sakalava Rail (Amaurornis olivieri). Conservation Genetics Resources 2 (Supp 1) , pp. 273-277. 10.1007/s12686-010-9189-2

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We isolated 76 microsatellite loci from the Sakalava rail (Amaurornis olivieri) genome. These were tested in 30 individuals from six sites (in three regions) across the species’ range in Madagascar. Thirty-four of the 76 loci produced unambiguous polymorphic markers, with mean H e = 0.544 (range 0.0678–0.885), mean H o = 0.430 (range 0.0400–0.767) and mean N a = 4.59 (range 2–10). In addition, we tested all primers for amplification in the Wood rail, Canirallus kioloides (N = 3) and the Bemaraha rail, Canirallus sp. nov (N = 3). Twelve of the loci produced successful amplification with eight loci showing polymorphism.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Sustainable Places Research Institute (PLACES)
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Uncontrolled Keywords: Amaurornis olivieri Canirallus kioloides; Rail; Waterbird; Madagascar; Microsatellite; Primer; Endangered
Publisher: Springer
ISSN: 1877-7252
Last Modified: 04 Jun 2017 03:18

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