The open reading frame of the Na+-dependent glutamate transporter GLAST-1 is expressed in bone and a splice variant of this molecule is expressed in bone and brain

Huggett, Jim, Mason, Deborah Jane ORCID: https://orcid.org/0000-0002-8666-6094 and Vaughan-Thomas, Anne 2000. The open reading frame of the Na+-dependent glutamate transporter GLAST-1 is expressed in bone and a splice variant of this molecule is expressed in bone and brain. FEBS Letters 485 (1) , pp. 13-18. 10.1016/S0014-5793(00)02175-X

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Abstract

Previously we have reported expression of an mRNA with homology to the Na+-dependent glutamate transporter, GLAST-1, in bone. Here we demonstrate that the complete open reading frame of GLAST-1 mRNA and corresponding 69 kDa protein are expressed in rat bone in vivo. We have also discovered a novel splice variant (GLAST-1a), lacking exon 3, expressed in rat bone and brain. A 55 kDa protein detected by anti-GLAST antibody in rat cerebellum corresponds to the molecular weight of unglycosylated GLAST-1a. This has led us to propose that GLAST-1a has an opposite orientation in the cell membrane to GLAST-1.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > Q Science (General)
Uncontrolled Keywords:	GLAST-1; bone; glutamate; transporter; splice variant
Publisher:	Elsevier
ISSN:	0014-5793
Last Modified:	19 Oct 2022 09:12
URI:	https://orca.cardiff.ac.uk/id/eprint/20254

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