Particle-induced oxidative damage is ameliorated by pulmonary antioxidants

Greenwell, Leona L., Moreno, Teresa, Jones, Timothy ORCID: https://orcid.org/0000-0002-4466-1260 and Richards, Roy J. 2002. Particle-induced oxidative damage is ameliorated by pulmonary antioxidants. Free Radical Biology and Medicine 32 (9) , pp. 898-905. 10.1016/S0891-5849(02)00782-7

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Abstract

This investigation focuses on the application of an in vitro assay in elucidating the role of lung lining fluid antioxidants in the protection against inhaled particles, and to investigate the source of bioreactivity in urban PM10 collections from South Wales. The Plasmid Assay is an in vitro method of assessing and comparing the oxidative bioreactivity of inhalable particles. This method has provided the basis of limited toxicological studies into various inhaled xenobiotics including asbestos, and more recently PM10. Carbon Black M120 and Diesel Exhaust Particles(DEP) were tested as PM10 surrogates, DEP displaying the greatest oxidative bioreactivity. Both urban PM2.5 (fine fraction) and PM2.5–10 (coarse fraction) (Cardiff, S. Wales, UK) caused significant damage, the coarse fraction displaying higher oxidative capacity. The soluble components were found to be responsible for most of the bioreactivity in both PM sizes. Low molecular components of fresh lung lavage were found to offer most antioxidant protection, and surrogate Epithelial Lining Fluid (sELF) showed significant amelioration of DNA damage by the coarse fraction but less effect against the fine. Overall, the coarse, soluble fraction of PM10 is a great source of oxidative bioreactivity, but natural pulmonary low molecular weight antioxidants can significantly ameliorate its effects.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Earth and Environmental Sciences
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH426 Genetics R Medicine > RC Internal medicine R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords:	PM10; PM2.5; Lung; Oxidative damage; Antioxidants; Free radicals
Publisher:	Elsevier
ISSN:	0891-5849
Last Modified:	05 Nov 2022 14:52
URI:	https://orca.cardiff.ac.uk/id/eprint/21833

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