A microfabricated graphitic carbon column for high performance liquid chromatography

Barrow, D. A. ORCID: https://orcid.org/0000-0003-2096-7262, Castell, O. K. ORCID: https://orcid.org/0000-0002-6059-8062, Sykes, N., Myers, P. and Ritchie, H. 2011. A microfabricated graphitic carbon column for high performance liquid chromatography. Journal of Chromatography A 1218 (15) , pp. 1983-1987. 10.1016/j.chroma.2010.11.086

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Abstract

We report the first development of a novel, planar, microfluidic, graphitic carbon separations column utilizing an array of graphitic micropillars of diamond cross-section as the chromatographic stationary phase. 795 nm femtosecond laser ablation was employed to subtractively machine fluidic architectures and a micropillared array in a planar, graphitic substrate as a monolithic structure. A sample injector was integrated on-chip, together with fluid-flow distribution architectures to minimize band-broadening and ensure sample equi-distribution across the micro-pillared column width. The separations chip was interfaced directly to the ESI probe of a Thermofisher Surveyor mass spectrometer, enabling the detection of test-mixture analytes following their differential retention on the micro-pillared graphitic column, thus demonstrating the exciting potential of this novel separations format. Importantly, unlike porous, graphitic microspheres, the temperature and pressure resilience of the microfluidic device potentially enables use in subcritical H2O chromatography.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	Q Science > QD Chemistry
Uncontrolled Keywords:	micropillars; graphitic carbon; HPLC; chromatography; chip; microfluidics; Femtosecond laser
Additional Information:	Selected Papers of 35th International Symposium on High Performance Liquid Phase Separations and Related Techniques
Publisher:	Elsevier
ISSN:	0021-9673
Last Modified:	05 Feb 2023 14:50
URI:	https://orca.cardiff.ac.uk/id/eprint/22065

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