Ultrastructural changes in the retinopathy, globe enlarged (rge) chick cornea

Boote, Craig ORCID: https://orcid.org/0000-0003-0348-6547, Hayes, Sally ORCID: https://orcid.org/0000-0001-8550-0108, Young, Robert David ORCID: https://orcid.org/0000-0002-8300-8002, Kamma-Lorger, Christina S., Hocking, Paul M., Elsheikh, Ahmed, Inglehearn, Chris F., Ali, Manir and Meek, Keith Michael Andrew ORCID: https://orcid.org/0000-0002-9948-7538 2009. Ultrastructural changes in the retinopathy, globe enlarged (rge) chick cornea. Journal of Structural Biology 166 (2) , pp. 195-204. 10.1016/j.jsb.2009.01.009

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Abstract

In the cornea, the precise organisation of fibrillar collagen and associated proteoglycans comprising the stromal extracellular matrix plays a major role in governing tissue form and function. Recently, abnormal collagen alignment was noted in the misshapen corneas of mature chickens affected by the retinopathy, globe enlarged (rge) mutation. Here we further characterize corneal ultrastructural changes as the rge eye develops post-hatch. Wide-angle X-ray scattering disclosed alteration to dominant collagen lamellae directions in the rge chick cornea, compared to age-matched controls. These changes accompanied eye globe enlargement and corneal flattening in affected birds, manifesting as a progressive loss of circumferential collagen alignment in the peripheral cornea and limbus in birds older than 1 month. Collagen intermolecular separation was unchanged in rge. However, small-angle X-ray scattering results suggest collagen fibril separation and diameter increase more rapidly towards the corneal periphery in rge at 3 months post-hatch compared to controls, although central collagen fibril diameter was unchanged. By transmission electron microscopy utilising cuprolinic blue stain, the morphology and distribution of stromal proteoglycans were unaltered in rge corneas otherwise demonstrating abnormal collagen fibril organisation. From a numerical simulation of tissue mechanics, progressive remodelling of stromal collagen in rge during globe enlargement post-hatch appears to be related to the corneal morphometric changes presented by the disease.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences
Subjects:	R Medicine > RE Ophthalmology
Uncontrolled Keywords:	Corneal stroma ; Chick ; Collagen fibril ; Biomechanics ; Proteoglycan
Publisher:	Elsevier
ISSN:	1047-8477
Last Modified:	19 Oct 2022 09:51
URI:	https://orca.cardiff.ac.uk/id/eprint/22438

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