Differential regulation of nuclear and cytosolic Ca2+ in HeLa cells

Badminton, Michael Norman, Campbell, Anthony Keith and Rembold, C. M. 1996. Differential regulation of nuclear and cytosolic Ca2+ in HeLa cells. Journal of Biological Chemistry 271 (49) , pp. 31210-31214. 10.1074/jbc.271.49.31210

Full text not available from this repository.

Abstract

The results reported in this study address the controversial issue that nuclear free Ca2+ ([Ca2+]n) may be regulated independently of cytosolic free Ca2+ ([Ca2+]c). We have measured [Ca2+]n and [Ca2+]c with recombinant aequorin targeted to the nucleus and cytosol in HeLa cells. We found that histamine, ATP, and ionomycin increased [Ca2+]c quantitatively more than [Ca2+]n, although the time course of these changes was similar. The difference between [Ca2+]c and [Ca2+]n depended on the stimulus, and the relative difference between [Ca2+]n and [Ca2+]c was less with ionomycin than with histamine or ATP. After depletion of the internal Ca2+ store, restoration of extracellular Ca2+ resulted in only increased [Ca2+]c without a significant increase in [Ca2+]n. Treatment with cyclopiazonic acid resulted in a delayed increases in [Ca2+]n compared to [Ca2+]c. These differences in both timing and magnitude of nuclear Ca2+ signals confirm that the cell can limit or delay increases in nuclear free Ca2+. Taken with the fact that an inositol phosphate signaling system resides in the nucleus and its envelope, our data support the hypothesis that [Ca2+]n may be independently regulated.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Pharmacy
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology R Medicine > RM Therapeutics. Pharmacology
Publisher:	American Society for Biochemistry and Molecular Biology
ISSN:	0021-9258
Last Modified:	05 Jun 2017 02:58
URI:	https://orca.cardiff.ac.uk/id/eprint/22509

Citation Data

Cited 89 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item