Comparison of transcription-mediated amplification and growth-based methods for the quantitation of Enterococcus bacteria in environmental waters

Morris, Ceri, Blackwood, A. D., Kirs, M., Buttigieg, Neil D., Morgan, Rhian R., Hogan, J. J., Weeks, Ian ORCID: https://orcid.org/0000-0002-6362-2929 and Noble, R. T. 2008. Comparison of transcription-mediated amplification and growth-based methods for the quantitation of Enterococcus bacteria in environmental waters. Applied and Environmental Microbiology 74 (10) , pp. 3319-3320. 10.1128/AEM.02623-07

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Abstract

An assay based on transcription-mediated amplification (TMA) technology was used to quantitate Enterococcus fecal indicator bacteria in environmental water samples. The results generated by this and two growth-based methods relative to the 104 most-probable-number or CFU-per-100-ml threshold show that the three methods are in good qualitative agreement when tested against a range of water samples taken from different locations. The results demonstrate sensitive and rapid detection (approximately 4 h from sample collection to result) and quantitation of Enterococcus bacteria compared to the results with the growth-based methods.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > Q Science (General)
Publisher:	ASM
ISSN:	0099-2240
Last Modified:	04 Jan 2023 02:05
URI:	https://orca.cardiff.ac.uk/id/eprint/23405

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