DPPC regulates COX-2 expression in monocytes via phosphorylation of CREB

Morris, R. H. K., Tonks, Amanda Jayne, Jones, K. P., Ahluwalia, M. K., Thomas, A. W., Tonks, Alex ORCID: https://orcid.org/0000-0002-6073-4976 and Jackson, S. K. 2008. DPPC regulates COX-2 expression in monocytes via phosphorylation of CREB. Biochemical and Biophysical Research Communications 370 (1) , pp. 174-178. 10.1016/j.bbrc.2008.03.052

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Abstract

The major phospholipid in pulmonary surfactant dipalmitoyl phosphatidylcholine (DPPC) has been shown to modulate inflammatory responses. Using human monocytes, this study demonstrates that DPPC significantly increased PGE(2) (P<0.05) production by 2.5-fold when compared to untreated monocyte controls. Mechanistically, this effect was concomitant with an increase in COX-2 expression which was abrogated in the presence of a COX-2 inhibitor. The regulation of COX-2 expression was independent of NF-kappaB activity. Further, DPPC increased the phosphorylation of the cyclic AMP response element binding protein (CREB; an important nuclear transcription factor important in regulating COX-2 expression). In addition, we also show that changing the fatty acid groups of PC (e.g. using l-alpha-phosphatidylcholine beta-arachidonoyl-gamma-palmitoyl (PAPC)) has a profound effect on the regulation of COX-2 expression and CREB activation. This study provides new evidence for the anti-inflammatory activity of DPPC and that this activity is at least in part mediated via CREB activation of COX-2.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	Pulmonary surfactant; Inflammation; DPPC; COX-2; CREB
Publisher:	Elsevier
ISSN:	0006-291X
Last Modified:	19 Oct 2022 10:17
URI:	https://orca.cardiff.ac.uk/id/eprint/23838

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