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First generation microarray-system for identification of primate species subject to bushmeat trade

Rönn, A., Andrés, O., López-Giráldez, F., Johnsson-Glans, C., Verschoor, E., Domingo-Roura, X., Bruford, Michael William, Syvänen, A. and Bosch, M. 2009. First generation microarray-system for identification of primate species subject to bushmeat trade. Endangered Species Research 9 (2) , pp. 133-142. 10.3354/esr00191

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About a quarter of non-human primate species are threatened by extinction in the near future. Loss of habitat, disease and illegal hunting, especially for the bushmeat trade, are major causes of concern. Here, we develop an identification tool for primate genera using diagnostic nucleotide positions in the epsilon globin gene, apolipoprotein B gene and mitochondrial 12S rRNA. We identified 111 diagnostic nucleotide positions suitable for genotyping by a minisequencing assay in a microarray format. To show the applicability of the microarray, we typed 70 non-human primates representing all primate infraorders. Sixty-five samples were assigned to the correct infraorder, and 32 were assigned to the correct genus (the highest level of taxonomic resolution attempted here). Our results show that it is feasible to distinguish among a high number of primate taxa if the system allows hierarchical assignation of the samples at different taxonomic levels and includes both taxon-specific and redundant positions.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Sustainable Places Research Institute (PLACES)
Subjects: Q Science > Q Science (General)
Q Science > QL Zoology
Uncontrolled Keywords: primates; bushmeat; minisequencing microarray; epsilon globin; apolipoprotein B; 12S rRNA
Publisher: Inter-Research
ISSN: 1863-5407
Last Modified: 04 Jun 2017 03:39

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