A novel function for a redox-related LEA protein (SAG21/AtLEA5) in root development and biotic stress responses

Mohd Salleh, Faeza, Evans, Karly, Goodall, Benjamin James, MacHin, Helena Christine, Mowla, Shaheen B., Mur, Luisa A. J., Runions, John, Theodoulou, Frederica L., Foyer, Christine H. and Rogers, Hilary Joan ORCID: https://orcid.org/0000-0003-3830-5857 2012. A novel function for a redox-related LEA protein (SAG21/AtLEA5) in root development and biotic stress responses. Plant, Cell & Environment 35 (2) , pp. 418-429. 10.1111/j.1365-3040.2011.02394.x

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Abstract

SAG21/AtLEA5 belongs to the late embryogenesis-associated (LEA) protein family. Although it has been implicated in growth and redox responses, its precise roles remain obscure. To address this problem, we characterized root and shoot development and response to biotic stress in SAG21/AtLEA5 over-expressor (OEX) and antisense (AS) lines. AS lines exhibited earlier flowering and senescence and reduced shoot biomass. Primary root length was reduced in AS lines, as was the number of laterals relative to the primary root. Root hair number was unchanged but root hair length was proportional to SAG21/AtLEA5 expression level, with longer root hairs in OEX lines and shorter root hairs in AS, relative to wild type. Growth of the fungal nectroph, Botrytis cinerea and of a virulent bacterial pathogen (Pseudomonas syringae pv. tomato) was affected by SAG21/AtLEA5 expression; however, growth of an avirulent P.syringae strain was unaffected. A SAG21/AtLEA5-YFP fusion was localized to mitochondria, raising the intriguing possibility that SAG21 interacts with proteins involved in mitochondrial ROS signalling, which in turn, impacts on root development and pathogen responses.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QK Botany
Uncontrolled Keywords:	Arabidopsis; disease; LEA proteins; mitochondria; redox signalling; ROS; root hair; senescence
Publisher:	Wiley-Blackwell
ISSN:	0140-7791
Last Modified:	19 Oct 2022 10:30
URI:	https://orca.cardiff.ac.uk/id/eprint/24587

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