WAVE1 is Associated with Invasiveness and Growth of Prostate Cancer Cells

Fernando, Herman S., Sanders, Andrew James ORCID: https://orcid.org/0000-0002-7997-5286, Kynaston, Howard ORCID: https://orcid.org/0000-0003-1902-9930 and Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111 2008. WAVE1 is Associated with Invasiveness and Growth of Prostate Cancer Cells. The Journal of Urology 180 (4) , pp. 1515-1521. 10.1016/j.juro.2008.06.004

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Abstract

Purpose WAVE1 belongs to the Wiskott-Aldrich syndrome family of proteins, which have an integral part in cell motility, a crucial step in cancer metastasis. We investigated the expression pattern and the effects of manipulating endogenous WAVE1 expression in prostatecancercells. Materials and Methods WAVE1 protein expression in normal and cancer specimens, and in prostatecell lines was assessed using immunohistochemistry and reverse transcriptase-polymerase chain reaction, respectively. Hammerhead ribozyme transgenes were synthesized and cloned into the mammalian expression vector pEF6/V5-His TOPO TA, and transfected by electroporation into PC-3ΔW1R1/2 and DU-145ΔW1R1/2cell lines. In vitro invasion, adhesion and growth assays were used to assess the impact of WAVE1 knockdown. Results Immunohistochemistry of prostate tissue specimens showed that the cytoplasm of cancercells had stronger staining than normal epithelium. Reverse transcriptase-polymerase chain reaction for WAVE1 showed strong expression in the PC-3 (European Collection of Cell Cultures, Salisbury, United Kingdom) and DU-145 (ATCC®) cell lines. WAVE1 knockdown was associated with a significant decrease in invasion but not in adhesion. The mean ± SEM number of invading PC-3ΔW1R1 and PC-3ΔW1R2cells was 7.27 ± 0.38 and 6 ± 0.29, respectively, compared to 12.27 ± 0.42 PC-3WTcells (p <0.001). Similarly the mean number of invading DU-145ΔW1R1 and DU-145ΔW1R2cells was 9.20 ± 0.70 and 11.60 ± 0.84 compared to 14.80 ± 0.24 DU-145WTcells (p <0.001). Conclusions To our knowledge this is the first report of the expression pattern of WAVE1 in prostatecancercell lines and tissues, and the functional impact of WAVE1 knockdow

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords:	prostate; prostatic neoplasms; WASF1 protein; human; Wiskott-Aldrich syndrome protein family; neoplasm metastasis
Publisher:	Elsevier
ISSN:	0022-5347
Last Modified:	19 Oct 2022 10:38
URI:	https://orca.cardiff.ac.uk/id/eprint/25053

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