EPLIN is a negative regulator of prostate cancer growth and invasion

Sanders, Andrew James ORCID: https://orcid.org/0000-0002-7997-5286, Martin, Tracey Amanda ORCID: https://orcid.org/0000-0003-2690-4908, Ye, Lin ORCID: https://orcid.org/0000-0002-0303-2409, Mason, Malcolm David ORCID: https://orcid.org/0000-0003-1505-2869 and Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111 2011. EPLIN is a negative regulator of prostate cancer growth and invasion. The Journal of Urology 186 (1) , pp. 295-301. 10.1016/j.juro.2011.03.038

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Abstract

PURPOSE: We investigated the importance of EPLIN, a cytoskeletal associated protein implicated in cancer, in clinical prostate cancer and its role in the PC-3 prostate cancer cell line (ATCC™). MATERIALS AND METHODS: Full-length human EPLIN cDNA was cloned into a pEF6 expression vector and used to transfect the PC-3 human prostate cancer cell line. Cells over expressing EPLIN were termed PC-3(EPLIN EXP) while wild-type and empty pEF6 vector control cells were designated PC-3(WT) and PC-3(pEF6), respectively. The in vitro and in vivo impact of EPLIN on PC-3 cells was examined using a number of model assays. RESULTS: EPLIN over expression in PC-3 cells resulted in a decrease in the growth rate of this cell line (mean ± SD 0.6 ± 0.17 for PC-3(pEF6) cells vs 0.33 ± 0.01 for PC-3(EPLIN EXP) cells, p <0.01). PC-3(EPLIN EXP) cells were significantly less able to adhere to extracellular matrix than control cells (mean 61.0 ± 12.4 vs 102.8 ± 20.7, p = 0.028). Immunofluorescence staining showed an increased staining profile for paxillin in PC-3(EPLIN EXP) cells compared to wild-type cells. CONCLUSIONS: EPLIN over expression in the PC-3 cell line resulted in decreased in vivo and in vitro growth potential together with decreased cell invasiveness and ability to adhere to extracellular matrix, and enhanced paxillin staining. This further highlights the importance of EPLIN in regulating prostate cancer cell growth and aggressiveness, and suggests a possible connection between EPLIN and paxillin.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords:	prostate; prostatic neoplasms; EPLIN protein; human; paxillin; neoplasm invasiveness
Publisher:	Elsevier
ISSN:	0022-5347
Last Modified:	07 Feb 2023 02:20
URI:	https://orca.cardiff.ac.uk/id/eprint/25281

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