Differential CARS microscopy with linearly chirped femtosecond laser pulses

Langbein, Wolfgang Werner ORCID: https://orcid.org/0000-0001-9786-1023, Rocha-Mendoza, Israel, Masia, Francesco ORCID: https://orcid.org/0000-0003-4958-410X, Di Napoli, Claudia, Pope, Iestyn ORCID: https://orcid.org/0000-0002-4104-0389, Watson, Peter Duncan ORCID: https://orcid.org/0000-0003-0250-7852 and Borri, Paola ORCID: https://orcid.org/0000-0002-7873-3314 2011. Differential CARS microscopy with linearly chirped femtosecond laser pulses. Presented at: Multiphoton Microscopy in the Biomedical Sciences XI, San Francisco, CA, USA, 23-25 January 2011. Published in: Periasamy, Ammasi, König, Karsten and So, Peter T. C. eds. Multiphoton Microscopy in the Biomedical Sciences XI. SPIE Proceedings , vol.7903 SPIE, 79031I. 10.1117/12.873872

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Abstract

We demonstrate frequency differential CARS (D-CARS) using femtosecond laser pulses linearly chirped by glass elements of high group-velocity dispersion. By replicating the Pump-Stokes pair into a pulse train at twice the laser repetition rate, and controlling the instantaneous frequency difference by glass dispersion, we adjust the Raman frequency probed by each pair in an intrinsically stable way. The resulting CARS intensities are detected simultaneously by a single photomultiplier as sum and difference using lock-in detection. We demonstrate imaging of living cells with strongly suppressed non-resonant background. We also show D-CARS using a single femtosecond laser source.

Item Type:	Conference or Workshop Item (Paper)
Date Type:	Publication
Status:	Published
Schools:	Biosciences Physics and Astronomy
Subjects:	Q Science > Q Science (General) T Technology > T Technology (General) T Technology > TA Engineering (General). Civil engineering (General) T Technology > TK Electrical engineering. Electronics Nuclear engineering
Publisher:	SPIE
ISBN:	9780819484406
ISSN:	0277-786X
Funders:	BBSRC, EPSRC
Last Modified:	14 Sep 2023 01:07
URI:	https://orca.cardiff.ac.uk/id/eprint/26010

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