Four-wave mixing of gold nanoparticles for three-dimensional cell microscopy

Langbein, Wolfgang Werner ORCID: https://orcid.org/0000-0001-9786-1023, Watson, Peter Duncan ORCID: https://orcid.org/0000-0003-0250-7852 and Borri, Paola ORCID: https://orcid.org/0000-0002-7873-3314 2009. Four-wave mixing of gold nanoparticles for three-dimensional cell microscopy. Presented at: Lasers and Electro-Optics 2009 and the European Quantum Electronics Conference. CLEO Europe - EQEC 2009. European Conference on, Munich, Germany, 14-19 June 2009. Published in: Masia, Francesco ORCID: https://orcid.org/0000-0003-4958-410X, Langbein, Wolfgang Werner ORCID: https://orcid.org/0000-0001-9786-1023, Watson, Peter Duncan ORCID: https://orcid.org/0000-0003-0250-7852 and Borri, Paola ORCID: https://orcid.org/0000-0002-7873-3314 eds. 2009 Conference on Lasers & Electro-Optics Europe & 11th European Quantum Electronics Conference (CLEO EUROPE/EQEC 2009). Institute of Electrical and Electronics Engineers ( IEEE ), 10.1109/CLEOE-EQEC.2009.5191714

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Abstract

Optical microscopy is an indispensable tool for obtaining spatial and temporal resolution within living cells and tissues. In this paper, a novel multiphoton microscopy technique which exploits the third-order nonlinearity called four-wave mixing and applied to gold nanoparticles in resonance with their surface plasmon is developed. The coherent, transient and resonant nature of this signal allows the interferometric time-resolved detection of four-wave mixing specifically originating from GNPs, with particle diameters down to 5nm. This paper demonstrate high-contrast, background-free imaging of gold-labelled Golgi structures in HepG2 cells with a sub-diffraction-limit lateral (axial) resolution of 140nm (470nm) at excitation powers corresponding to less than 3K photothermal heating, compatible to live cell imaging. These results pave the way towards photostable non-toxic and highly sensitive in-vivo optical imaging with sub-micron three-dimensional resolution.

Item Type:	Conference or Workshop Item (Paper)
Date Type:	Publication
Status:	Published
Schools:	Schools > Biosciences Schools > Physics and Astronomy
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology T Technology > T Technology (General) T Technology > TA Engineering (General). Civil engineering (General) T Technology > TK Electrical engineering. Electronics Nuclear engineering
Publisher:	Institute of Electrical and Electronics Engineers ( IEEE )
ISBN:	9781424440795
Last Modified:	06 Jul 2024 11:14
URI:	https://orca.cardiff.ac.uk/id/eprint/27008

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