The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B

Rosas, Marcela ORCID: https://orcid.org/0000-0002-9442-9638, Thomas, Benjamin, Stacey, Martin, Gordon, Siamon and Taylor, Philip Russel ORCID: https://orcid.org/0000-0003-0163-1421 2010. The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B. Journal of leukocyte biology 88 (1) , pp. 169-180. 10.1189/jlb.0809548

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Abstract

This study aimed to identify the inflammation-associated 7/4-antigen, which is highly expressed on neutrophils, inflammatory monocytes, some activated macrophages, as well as on bone marrow myeloid-restricted progenitors. The high expression on inflammatory cells is suggestive of a role in inflammation and makes the 7/4-antigen a potential target for the manipulation of inflammatory cells. Consistent with this, the 7/4-antibody mediates specific depletion of 7/4-expressing neutrophils and monocytes. We have identified the 7/4-antigen as a 25- to 30-kDa GPI-anchored glycoprotein synonymous with the Ly-6B.2 alloantigen. We characterized the expression of Ly-6B during the inflammatory reaction induced by zymosan. During the later stages of an experimental, acute, self-resolving inflammatory response, we found that Ly-6B is differentially expressed on macrophages. Ly-6B-expressing macrophages also express more MHCII, CIITA, CCR2, Ly-6C, and CD62L than the Ly-6B-negative macrophages, which in turn, express more of the resident tissue macrophage marker SIGN-R1 and higher CD11b and F4/80. Ly-6B-expressing macrophages incorporate more BrdU than their Ly-6B-negative contemporaries when fed during the resolution phase of the acute inflammatory response. Thus, Ly-6B expression on mature macrophages defines a subset of recently generated inflammatory macrophages that retain monocytic markers and is hence a surrogate marker of macrophage turnover in inflammatory lesions. The definition of the 7/4:Ly-6B antigen will allow further characterization and specific modulation of Ly-6B-expressing cells in vivo.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > QR Microbiology > QR180 Immunology R Medicine > R Medicine (General)
Uncontrolled Keywords:	Monocyte ; Surface antigen ; Inflammation ; u-PAR
Publisher:	Society for leukocyte biology
ISSN:	0741-5400
Last Modified:	20 Oct 2022 08:27
URI:	https://orca.cardiff.ac.uk/id/eprint/28433

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