Distribution and expression of picalm in alzheimer disease

Baig, Shabnam, Joseph, Sally A., Tayler, Hannah, Abraham, Richard Alun, Owen, Michael John ORCID: https://orcid.org/0000-0003-4798-0862, Williams, Julie ORCID: https://orcid.org/0000-0002-4069-0259, Kehoe, Patrick G. and Love, Seth 2010. Distribution and expression of picalm in alzheimer disease. Journal of Neuropathology and Experimental Neurology 69 (10) , pp. 1071-1077. 10.1097/NEN.0b013e3181f52e01

Full text not available from this repository.

Abstract

PICALM, the gene encoding phosphatidylinositol-binding clathrin assembly (picalm) protein, was recently shown to be associated with risk of Alzheimer disease (AD). Picalm is a key component of clathrin-mediated endocytosis. It recruits clathrin and adaptor protein 2 (AP-2) to the plasma membrane and, along with, AP-2 recognizes target proteins. The attached clathrin triskelions cause membrane deformation around the target proteins enclosing them within clathrin-coated vesicles to be processed in lysosomes or endosomes. We examined the distribution of picalm in control and AD brain tissue and measured levels of picalm messenger RNA (mRNA) by real-time polymerase chain reaction. Immunolabeling of brain tissue showed that picalm is predominately present in endothelial cells. This was further supported by the demonstration of picalm in human cerebral microvascular cells grown in culture. Picalm mRNA was elevated in relation to glyceraldehyde-3-phosphate dehydrogenase but not factor VIII-related antigen or CD31 mRNA in the frontal cortex in AD. No change was seen in the temporal cortex or thalamus. The transport of Aβ across vessel walls and into the bloodstream is a major pathway of Aβ removal from the brain and picalm is ideally situated within endothelial cells to participate in this process. Further research is needed to determine whether PICALM expression is influenced by Aβ levels and whether it affects Aβ uptake and transport by endothelial cells.

Item Type:	Article
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI) MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG) Neuroscience and Mental Health Research Institute (NMHRI)
Subjects:	R Medicine > R Medicine (General)
Publisher:	Lippincott Williams & Wilkins
ISSN:	0022-3069
Last Modified:	20 Oct 2022 08:36
URI:	https://orca.cardiff.ac.uk/id/eprint/28918

Citation Data

Cited 80 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item