Development of FRET-Based assays in the Far-Red using CdTe quantum dots

Chong, E. Z., Matthews, D. R., Summers, H. D., Njoh, Kerenza, Errington, Rachel Jane ORCID: https://orcid.org/0000-0002-8016-4376 and Smith, Paul James 2007. Development of FRET-Based assays in the Far-Red using CdTe quantum dots. Journal of Biomedicine and Biotechnology 2007 , 54169. 10.1155/2007/54169

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Abstract

Colloidal quantum dots (QDs) are now commercially available in a biofunctionalized form, and Förster resonance energy transfer (FRET) between bioconjugated dots and fluorophores within the visible range has been observed. We are particularly interested in the far-red region, as from a biological perspective there are benefits in pushing to ∼700 nm to minimize optical absorption (ABS) within tissue and to avoid cell autofluorescence. We report on FRET between streptavidin- (STV-) conjugated CdTe quantum dots, Qdot705-STV, with biotinylated DY731-Bio fluorophores in a donor-acceptor assay. We also highlight the changes in DY731-Bio absorptivity during the streptavidin-biotin binding process which can be attributed to the structural reorientation. For fluorescence beyond 700 nm, different alloy compositions are required for the QD core and these changes directly affect the fluorescence decay dynamics producing a marked biexponential decay with a long-lifetime component in excess of 100 nanoseconds. We compare the influence of the two QD relaxation routes upon FRET dynamics in the presence of DY731-Bio.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	Q Science > QH Natural history > QH426 Genetics R Medicine > R Medicine (General)
Publisher:	Hindawi
ISSN:	1110-7243
Date of First Compliant Deposit:	30 March 2016
Last Modified:	05 May 2023 00:22
URI:	https://orca.cardiff.ac.uk/id/eprint/37100

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