Cell-population tracking using quantum dots in flow cytometry

Summers, Huw D., Errington, Rachel Jane ORCID: https://orcid.org/0000-0002-8016-4376, Smith, Paul James, Chappell, Sally Claire, Rees, Paul, Brown, Martyn R. and Leary, James F. 2008. Cell-population tracking using quantum dots in flow cytometry. Presented at: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications V, San Jose, CA, USA, 21-23 February 2008. Published in: Cartwright, A. N. and Nicolau, D. V. eds. Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications V. Proceedings of the SPIE (6865) Bellingham, WA: SPIE, 68650L. 10.1117/12.763602

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Abstract

We have used flow-cytometry together with computational modeling of quantum dot portioning during cell division to identify population distributions of proliferating cells. The objective has been to develop a robust assay of integrated cellular fluorescence which reports the extent of cellular bifurcation within a complex population and potentially provides profiles of drug resistance, cell clonality and levels of aneuploidy in tumour cells. The implementation of a data analysis program based on genetic algorithms provides a complete description of the proliferation dynamics and gives values for the inter-mitotic time, the partitioning ratio of quantum dots between daughter cells and their associated deviations.

Item Type:	Conference or Workshop Item (Paper)
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	Q Science > QH Natural history > QH301 Biology R Medicine > R Medicine (General)
Publisher:	SPIE
ISBN:	9780819470409
Last Modified:	21 Oct 2022 09:37
URI:	https://orca.cardiff.ac.uk/id/eprint/37103

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