A robust plate assay for detection of extracellular microbial protease activity in metagenomic screens and pure cultures

Morris, Laura, Evans, James and Marchesi, Julian Roberto ORCID: https://orcid.org/0000-0002-7994-5239 2012. A robust plate assay for detection of extracellular microbial protease activity in metagenomic screens and pure cultures. Journal of Microbiological Methods 91 (1) , pp. 144-146. 10.1016/j.mimet.2012.08.006

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Abstract

A robust, efficient and cost-effective agar that utilises lactose free milk powder for identification of bacterial protease activity in pure cultures and metagenomic screens has been developed and tested on protease positive bacteria, selected strains and false protease positives isolated from a previously constructed metagenomic library.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > Q Science (General) Q Science > QR Microbiology
Publisher:	Elsevier
ISSN:	0167-7012
Last Modified:	21 Oct 2022 10:56
URI:	https://orca.cardiff.ac.uk/id/eprint/41797

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