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Association of differentiation-related gene-1 (DRG1) with breast cancer survival and in vitro impact of DRG1 suppression

Baig, Ruqia Mehmood, Sanders, Andrew James

, Kayani, Mahmood Akhtar and Jiang, Wen Guo

2012. Association of differentiation-related gene-1 (DRG1) with breast cancer survival and in vitro impact of DRG1 suppression. Cancers 4 (3) , pp. 658-672. 10.3390/cancers4030658

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Official URL: http://dx.doi.org/10.3390/cancers4030658

Abstract

Differentiation-related gene-1, DRG1, is a metastasis suppressor gene whose expression has been shown to be dysregulated in a number of malignancies. The current study examines the expression of DRG1 in a clinical breast cohort and its association with a number of clinical pathological factors using quantitative polymerase chain reaction. Additionally, DRG1 expression is targeted in vitro using ribozyme transgene technology to explore the function of DRG1 in two human breast cancer cell lines. Low levels of DRG1 were found in patients who developed metastasis (p = 0.036) and who died of breast cancer (p = 0.0048) compared to disease free patients. Knockdown of DRG1 also resulted in significantly increased invasion and motility, but decreased matrix-adhesion in MCF7 cells. Knockdown of DRG1 seemed to have minimal impact on the cellular functions of the MDA-MB-231 breast cancer cell line causing no significant differences in cell growth, invasion, motility or matrix-adhesion. Thus, DRG1 appears to be linked to development of metastasis and death in patients who died as a result of breast cancer and may be useful as a prognostic factor as its knockdown appears to be linked with increased invasion and motility and decreased adhesion in MCF7 breast cancer cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	Q Science > QH Natural history > QH426 Genetics R Medicine > R Medicine (General) R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords:	DRG1; breast cancer; metastasis; prognosis
Additional Information:	Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/2072-6694/ (accessed 20/02/2014).
Publisher:	MDPI AG
ISSN:	2072-6694
Date of First Compliant Deposit:	30 March 2016
Last Modified:	05 May 2023 08:04
URI:	https://orca.cardiff.ac.uk/id/eprint/43283