Phospholipase Cζ causes Ca2+ oscillations and parthenogenetic activation of human oocytes

Rogers, N. T., Hobson, E., Pickering, S., Lai, Francis Anthony ORCID: https://orcid.org/0000-0003-2852-8547, Braude, P. and Swann, Karl ORCID: https://orcid.org/0000-0002-4355-1449 2004. Phospholipase Cζ causes Ca2+ oscillations and parthenogenetic activation of human oocytes. Reproduction 128 (6) , pp. 697-702. 10.1530/rep.1.00484

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Abstract

At fertilization in mammals the sperm activates development of the oocyte by inducing a prolonged series of oscillations in the cytosolic free Ca2+ concentration. One theory of signal transduction at fertilization suggests that the sperm cause the Ca2+ oscillations by introducing a protein factor into the oocyte after gamete membrane fusion. We recently identified this sperm-specific protein as phospholipase Cζ (PLCζ), and we showed that PLCζ triggers Ca2+ oscillations in unfertilized mouse oocytes. Here we report that microinjection of the complementary RNA for human PLCζ causes prolonged Ca2+ oscillations in aged human oocytes that had failed to fertilize during in vitro fertilization or intracytoplasmic sperm injection. The frequency of Ca2+ oscillations was related to the concentration of complementary RNA injected. At low concentrations, PLCζ stimulated parthenogenetic activation of oocytes. These embryos underwent cleavage divisions and some formed blastocysts. These data show that PLCζ is a novel parthenogenetic stimulus for human oocytes and that it is unique in its ability to mimic the repetitive nature of the Ca2+ stimulus provided by the sperm during human fertilization.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology
Publisher:	Society for Reproduction and Fertility
ISSN:	1470-1626
Last Modified:	24 Oct 2022 10:55
URI:	https://orca.cardiff.ac.uk/id/eprint/46407

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