Interaction with surrounding normal epithelial cells influences signalling pathways and behaviour of Src-transformed cells

Kajita, Mihoko, Hogan, Catherine ORCID: https://orcid.org/0000-0002-1012-0896, Harris, Andrew R., Dupre-Crochet, Sophie, Itasaki, Nobue, Kawakami, Koichi, Charras, Guillaume, Tada, Masazumi and Fujita, Yasuyuki 2010. Interaction with surrounding normal epithelial cells influences signalling pathways and behaviour of Src-transformed cells. Journal of Cell Science 123 (2) , pp. 171-180. 10.1242/jcs.057976

Full text not available from this repository.

Abstract

At the initial stage of carcinogenesis, transformation occurs in a single cell within an epithelial sheet. However, it remains unknown what happens at the boundary between normal and transformed cells. Using Madin-Darby canine kidney (MDCK) cells transformed with temperature-sensitive v-Src, we have examined the interface between normal and Src-transformed epithelial cells. We show that Src-transformed cells are apically extruded when surrounded by normal cells, but not when Src cells alone are cultured, suggesting that apical extrusion occurs in a cell-context-dependent manner. We also observe apical extrusion of Src-transformed cells in the enveloping layer of zebrafish gastrula embryos. When Src-transformed MDCK cells are surrounded by normal MDCK cells, myosin-II and focal adhesion kinase (FAK) are activated in Src cells, which further activate downstream mitogen-activated protein kinase (MAPK). Importantly, activation of these signalling pathways depends on the presence of surrounding normal cells and plays a crucial role in apical extrusion of Src cells. Collectively, these results indicate that interaction with surrounding normal epithelial cells influences the signalling pathways and behaviour of Src-transformed cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences European Cancer Stem Cell Research Institute (ECSCRI)
Subjects:	Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords:	Apical extrusion; Focal adhesion kinase; Mitogen-activated protein kinase; Myosin-II; Src; Zebrafish
Publisher:	The Company of Biologists
ISSN:	0021-9533
Last Modified:	25 Oct 2022 08:01
URI:	https://orca.cardiff.ac.uk/id/eprint/51130

Citation Data

Cited 144 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item