Dynamic and reversible control of 2D membrane protein concentration in a droplet interface bilayer

Gross, Linda C. M., Castell, Oliver Kieran ORCID: https://orcid.org/0000-0002-6059-8062 and Wallace, Mark I. 2011. Dynamic and reversible control of 2D membrane protein concentration in a droplet interface bilayer. Nano Letters 11 (8) , pp. 3324-3328. 10.1021/nl201689v

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Abstract

We form an artificial lipid bilayer between a nanolitre aqueous droplet and a supporting hydrogel immersed in an oil/lipid solution. Manipulation of the axial position of the droplet relative to the hydrogel controls the size of the bilayer formed at the interface; this enables the surface density of integral membrane proteins to be controlled. We are able to modulate the surface density of the β-barrel pore-forming toxin α-hemolysin over a range of 4 orders of magnitude within a time frame of a few seconds. The concentration changes are fully reversible. Membrane protein function and diffusion are unaltered, as measured by single molecule microscopy and single channel electrical recording.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Pharmacy
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords:	Droplet interface bilayer; molecular crowding; protein−protein interaction; protein concentration
Publisher:	American Chemical Society
ISSN:	1530-6984
Last Modified:	25 Oct 2022 08:04
URI:	https://orca.cardiff.ac.uk/id/eprint/51312

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