Identification and characterization of tumor-initiating cells in human primary cutaneous squamous cell carcinoma

Patel, Girish Khandubhai, Yee, Carole L., Terunuma, Atsushi, Telford, William G., Voong, Nga, Yuspa, Stuart H. and Vogel, Jonathan C. 2012. Identification and characterization of tumor-initiating cells in human primary cutaneous squamous cell carcinoma. Journal of Investigative Dermatology 132 (2) , pp. 401-409. 10.1038/jid.2011.317

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Abstract

Primary human squamous cell carcinomas (SCCas) are heterogeneous invasive tumors with proliferating outer layers and inner differentiating cell masses. To determine if tumor-initiating cells (TICs) are present in SCCas, we utilized newly developed reliable in vitro and in vivo xenograft assays that propagate human SCCas, and demonstrated that a small subset of SCCa cells (~1%) expressing Prominin-1 (CD133) in the outer layers of SCCas were highly enriched for TICs (~1/400) compared with unsorted SCCa cells (TICs ~1/106). Xenografts of CD133+ SCCas recreated the original SCCa tumor histology and organizational hierarchy, whereas CD133− cells did not, and only CD133+ cells demonstrated the capacity for self-renewal in serial transplantation studies. We present a model of human SCCas in which tumor projections expand with outer leading edges that contain CD133+ TICs. Successful cancer treatment will likely require that the TICs identified in cancers be targeted therapeutically. The demonstration that TICs are present in SCCas and are enriched in a CD133− expressing subpopulation has not been, to our knowledge, previously reported.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences European Cancer Stem Cell Research Institute (ECSCRI)
Subjects:	Q Science > QH Natural history > QH301 Biology R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) R Medicine > RL Dermatology
Publisher:	Nature Publishing Group
ISSN:	0022-202X
Last Modified:	04 Jun 2017 05:33
URI:	https://orca.cardiff.ac.uk/id/eprint/51907

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