A spindle assembly checkpoint protein functions in prophase I arrest and prometaphase progression

Homer, Hayden, Gui, Liming and Carroll, John 2009. A spindle assembly checkpoint protein functions in prophase I arrest and prometaphase progression. Science 326 (5955) , pp. 991-994. 10.1126/science.1175326

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Abstract

Two critical stages of mammalian oocyte regulation are prophase I arrest, which is important for sustaining the oocyte pool, and the progression through meiosis I (MI) to produce fertilizable eggs. We have found that the spindle assembly checkpoint protein BubR1 regulates both stages in mouse oocytes. We show that oocytes depleted of BubR1 cannot sustain prophase I arrest and readily undergo germinal vesicle breakdown, a marker for reentry into MI. BubR1-depleted oocytes then arrest before completing MI, marked by failure of polar body extrusion. Both meiotic defects in BubR1-depleted oocytes are due to reduced activity of the master regulator known as the anaphase-promoting complex (APC), brought about through diminished levels of the APC coactivator Cdh1.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences European Cancer Stem Cell Research Institute (ECSCRI)
Subjects:	Q Science > QH Natural history > QH301 Biology
Publisher:	American Association for the Advancement of Science
ISSN:	0036-8075
Last Modified:	28 Jun 2019 02:02
URI:	https://orca.cardiff.ac.uk/id/eprint/51912

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