'Bacillus thuringiensis' colonises plant roots in a phylogeny-dependent manner

Vidal Quist, Jose, Rogers, Hilary Joan ORCID: https://orcid.org/0000-0003-3830-5857, Mahenthiralingam, Eshwar ORCID: https://orcid.org/0000-0001-9014-3790 and Berry, Colin ORCID: https://orcid.org/0000-0002-9943-548X 2013. 'Bacillus thuringiensis' colonises plant roots in a phylogeny-dependent manner. FEMS Microbiology Ecology 86 (3) , pp. 474-489. 10.1111/1574-6941.12175

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Abstract

Although much is known about the pathology of Bacillus thuringiensis against invertebrates, current understanding of its natural ecology is limited. This study evaluated the biodiversity of B. thuringiensis in relation to its interaction with plants. Phylogenetic relationships between 44 reference and field-collected strains, determined using 16S rRNA and gyrB gene sequences, revealed a high degree of variability, similar to that found in databases. An Arabidopsis thaliana in vitro inoculation model was developed to screen the ability of B. thuringiensis to colonise roots. Significant colonisation differences up to 91-fold were observed between strains, and correlation between strain phylogeny and colonisation was found. The genetics and biochemistry of auxin production; presence of the gene encoding indole pyruvate decarboxylase; and the abilities of Bt strains to swarm, grow in rich/minimal media and affect root growth differed between the strains, but only auxin production correlated significantly with ability to colonise roots. Co-inoculation with Burkholderia phytofirmans PsJN or Pseudomonas fluorescens SBW25 produced no effect on B. thuringiensis colonisation levels, regardless of the co-inoculant. Similarly, root colonisation of A. thaliana mutants impaired in plant defences was not significantly higher compared with controls. This is the first systematic and phylogenetic evaluation of B. thuringiensis interaction with plants.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QR Microbiology
Uncontrolled Keywords:	Arabidopsis thaliana; gyrB; colonisation; inoculation; endophyte; rhizobacteria
Additional Information:	Online publication date: 25 July 2013.
Publisher:	Wiley-Blackwell
ISSN:	0168-6496
Last Modified:	25 Oct 2022 08:34
URI:	https://orca.cardiff.ac.uk/id/eprint/53445

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