Multigenotype HCV-NS3 recombinant vaccinia viruses as a model for evaluation of cross-genotype immunity induced by HCV vaccines in the mouse

Eisenbach, Christoph, Freyse, Anne, Lupu, Catalin M., Weigand, Kilian, Ernst, Evelyn, Hoyler, Birgit, Stremmel, Wolfgang, Bugert, Joachim Jakob ORCID: https://orcid.org/0000-0002-0556-3211 and Encke, Jens 2006. Multigenotype HCV-NS3 recombinant vaccinia viruses as a model for evaluation of cross-genotype immunity induced by HCV vaccines in the mouse. Vaccine 24 (24) , pp. 5140-5148. 10.1016/j.vaccine.2006.04.013

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Abstract

Surrogate infections with HCV-recombinant vaccinia viruses (HCV-rVV) are a standard method to test the efficacy of hepatitis C virus (HCV) vaccine candidates in the mouse model. We established a panel of 16 HCV-rVV expressing the nonstructural protein 3 (NS3) of HCV genotypes 1a, 1b, 2, 3 and 4. Mice immunized with recombinant NS3 protein derived from HCV genotype 1b were challenged with the rVV. rVV-titers decreased up to 54-fold after subtype 1b challenge and up to 8.5-fold after subtype 1a challenge. No change was detected for genotype 2, 3, or 4. Our model is a convenient and reliable tool to analyze the induction of cross-genotype immunity by experimental vaccination of mice.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
ISSN:	0264-410X
Last Modified:	25 Oct 2022 09:14
URI:	https://orca.cardiff.ac.uk/id/eprint/57747

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