Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens

Melquiot, Nadja V. and Bugert, Joachim Jakob ORCID: https://orcid.org/0000-0002-0556-3211 2004. Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens. Isaacs, Stuart N., ed. Vaccinia Virus and Poxvirology: Methods and Protocols, Methods in Molecular Biology, vol. 269. Totowa, NJ.: Humana Press, pp. 371-383. (10.1385/1-59259-789-0:371)

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Abstract

Molluscum contagiosum virus (MCV) lesions are limited to the human epidermis and can persist for months, showing only weak signs of inflammation. Because a culture system has not yet been created to replicate MCV, this chapter describes how the virus can be isolated from patient specimens. From this material, we describe how MCV is purified and used for infection studies, electron microscopy, viral DNA extraction, and analyses of early mRNA synthesized by in vitro transcription of permeabilized virions. The complete MCV-1 genome has been sequenced, and a redundant MCV genome fragment library of MCV type 1 (available from American Type Culture Collection [ATCC]) is useful for the cloning and study of individual MCV genes.

Item Type:	Book Section
Book Type:	Scholarly Edition
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Humana Press
ISBN:	9781588292292
Last Modified:	25 Oct 2022 09:14
URI:	https://orca.cardiff.ac.uk/id/eprint/57748

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