Macrorestriction fingerprinting of "Streptococcus milleri" group bacteria by pulsed-field gel electrophoresis

Bartie, Kerry L., Wilson, Melanie ORCID: https://orcid.org/0000-0002-8834-8268, Williams, David Wynne ORCID: https://orcid.org/0000-0002-7351-5131 and Lewis, Michael Alexander Oxenham ORCID: https://orcid.org/0000-0003-1917-0651 2000. Macrorestriction fingerprinting of "Streptococcus milleri" group bacteria by pulsed-field gel electrophoresis. Journal of Clinical Microbiology 38 (6) , pp. 2141-2149.

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Abstract

Although isolates of the “Streptococcus milleri” group (SMG) of bacteria are regarded as members of the commensal microflora of the body, they are frequently encountered in purulent infections from a range of body sites. The genetic diversity of 91 epidemiologically unrelated SMG isolates (including 37 commensal strains and 49 disease-associated strains) was analyzed by macrorestriction fingerprinting (MF). The genomes were digested withSmaI and ApaI independently, and fragments were resolved by pulsed-field gel electrophoresis. Similarities between banding profiles were determined, and strains were clustered on this basis into dendrograms. In common with other commensal species that have been examined by MF, considerable genetic diversity was revealed. In addition, the clustering of strains tended to support the current taxonomic position of this heterogeneous group. The present study has shown that MF is a powerful tool for characterization of SMG strains and that its use is likely to be of great value in epidemiological and population genetic studies of this group of bacteria.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Subjects:	R Medicine > RK Dentistry
Publisher:	American Society for Microbiology
ISSN:	0095-1137
Last Modified:	25 Oct 2022 10:04
URI:	https://orca.cardiff.ac.uk/id/eprint/61069

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