Heisenberg, C. -P., Houart, Corinne, Take-uchi, Masaya, Rauch, Gerd-Jörg, Young, Neville, Coutinho, Pedro, Masai, Ichiro, Caneparo, Luca, Concha, Miguel L., Geisler, Robert, Dale, Trevor Clive ![]() |
Abstract
Zebrafish embryos homozygous for the masterblind(mbl) mutation exhibit a striking phenotype in which the eyes and telencephalon are reduced or absent and diencephalic fates expand to the front of the brain. Here we show that mbl −/−embryos carry an amino-acid change at a conserved site in the Wnt pathway scaffolding protein, Axin1. The amino-acid substitution present in the mbl allele abolishes the binding of Axin to Gsk3 and affects Tcf-dependent transcription. Therefore, Gsk3 activity may be decreased in mbl −/− embryos and in support of this possibility, overexpression of either wild-type Axin1 or Gsk3β can restore eye and telencephalic fates to mbl −/−embryos. Our data reveal a crucial role for Axin1-dependent inhibition of the Wnt pathway in the early regional subdivision of the anterior neural plate into telencephalic, diencephalic, and eye-forming territories.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Schools > Biosciences Research Institutes & Centres > European Cancer Stem Cell Research Institute (ECSCRI) |
ISSN: | 0890-9369 |
Funders: | Cancer Research UK |
Last Modified: | 22 Jun 2023 10:01 |
URI: | https://orca.cardiff.ac.uk/id/eprint/61738 |
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