Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Variation in the DNA methylation pattern of expressed and nonexpressed genes in chicken

Cooper, David Neil, Errington, L. H. and Clayton, R. M. 1983. Variation in the DNA methylation pattern of expressed and nonexpressed genes in chicken. DNA 2 (2) , pp. 131-140. 10.1089/dna.1983.2.131

Full text not available from this repository.


Using methyl-sensitive and -insenstive restriction enzymes, Hpa II and Msp I, the methylation status of various chicken genes was examined in different tissues and developmental stages. Tissue-specific differences in methylation were found for the δ-crystallin, β-tubulin, G3PDH, rDNA, and actin genes but not for the histone genes. Developmental decreases in methylation were noted for the δ-crystallin and actin genes in chicken kidney between embryo and adult. Since most of the sequences examined were housekeeping genes, transcriptional differences are apparently not a necessary accompaniment to changes in DNA methylation at the CpG sites examined. The only exception is sperm DNA where the δ-crystallin, β-tubulin, and actin genes are highly methylated and almost certainly not transcribed. However, the G3PDH genes are no more highly methylated in sperm than in other somatic tissues. Many sequences homologous to the rDNA and histone probes used are unmethylated in all tissues examined including sperm, but a methylated rDNA subfraction is more heavily methylated in sperm than in other tissues. We speculate as to the significance of these differences in sperm DNA methylation in the light of possible requirements for early gene activation and the probable deleterious mutagenic effects of heavy methylation within coding sequences.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Mary Ann Liebert
ISSN: 0198-0238
Last Modified: 04 Jun 2017 06:33

Citation Data

Cited 11 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item