Two ENU-induced mutations in Rasgrf1 and early mouse growth retardation

Clapcott, Steven J., Peters, Josephine, Orban, Paul C., Brambilla, Riccardo ORCID: https://orcid.org/0000-0003-3569-5706 and Graham, Chris F. 2003. Two ENU-induced mutations in Rasgrf1 and early mouse growth retardation. Mammalian Genome 14 (8) , pp. 495-505. 10.1007/s00335-002-2258-4

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Abstract

When paternally transmitted, two independent ENU-induced mutations showed reduced whole body wet weight soon after birth. The mutations were mapped to Chromosome 9 (Chr 9) between the markers D9Mit208 and D9Mit215. Their map position and imprinted status suggested that they might alter RAS protein-specific guanine nucleotide releasing factor 1 expression. Both mutations introduced premature chain termination codons into the coding sequence of Rasgrf1, and no Ras-GRF1 protein was detected in the brain. The GENA53 line had a C to T transition at nucleotide 2137, and the line GENA37 had a T to A transversion at nucleotide 3552 of the cDNA sequence. Mutant mice had near normal body weight at birth, but their weight started to lag behind that of wild-type littermates during the first week, and they were about 15% lighter as adults.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Biosciences
Subjects:	Q Science > QH Natural history Q Science > QH Natural history > QH426 Genetics
Publisher:	Springer Verlag
ISSN:	0938-8990
Last Modified:	27 Oct 2022 08:26
URI:	https://orca.cardiff.ac.uk/id/eprint/62311

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