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Two ENU-induced mutations in Rasgrf1 and early mouse growth retardation

Clapcott, Steven J., Peters, Josephine, Orban, Paul C., Brambilla, Riccardo and Graham, Chris F. 2003. Two ENU-induced mutations in Rasgrf1 and early mouse growth retardation. Mammalian Genome 14 (8) , pp. 495-505. 10.1007/s00335-002-2258-4

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When paternally transmitted, two independent ENU-induced mutations showed reduced whole body wet weight soon after birth. The mutations were mapped to Chromosome 9 (Chr 9) between the markers D9Mit208 and D9Mit215. Their map position and imprinted status suggested that they might alter RAS protein-specific guanine nucleotide releasing factor 1 expression. Both mutations introduced premature chain termination codons into the coding sequence of Rasgrf1, and no Ras-GRF1 protein was detected in the brain. The GENA53 line had a C to T transition at nucleotide 2137, and the line GENA37 had a T to A transversion at nucleotide 3552 of the cDNA sequence. Mutant mice had near normal body weight at birth, but their weight started to lag behind that of wild-type littermates during the first week, and they were about 15% lighter as adults.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history
Q Science > QH Natural history > QH426 Genetics
Publisher: Springer Verlag
ISSN: 0938-8990
Last Modified: 04 Jun 2017 06:34

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