Involvement of regulatory volume decrease in the migration of nasopharyngeal carcinoma cells

Mao, Jian Wen, Wang, Liwei, Jacob, Timothy John Claud, Sun, Xue Rong, Li, Hui, Zhu, Lin Yan, Li, Pan, Zhong, Ping, Nie, Si Huai and Chen, Lixin 2005. Involvement of regulatory volume decrease in the migration of nasopharyngeal carcinoma cells. Cell Research 15 (5) , pp. 371-378. 10.1038/sj.cr.7290304

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Abstract

The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (CNE-2Z cells). Both migrated and non-migrated CNE-2Z cells, when swollen by 47% hypotonic solution, exhibited RVD which was inhibited by extracellular application of chloride channel blockers adenosine 5'-triphosphate (ATP), 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and tamoxifen. However, RVD rate in migrated CNE-2Z cells was bigger than that of non-migrated cells and the sensitivity of migrated cells to NPPB and tamoxifen was higher than that of non-migrated cells. ATP, NPPB and tamoxifen also inhibited migration of CNE-2Z cells. The inhibition of migration was positively correlated to the blockage of RVD, with a correlation coefficient (r) = 0.99, suggesting a functional relationship between RVD and cell migration. We conclude that RVD is involved in cell migration and RVD may play an important role in migratory process in CNE-2Z cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > Q Science (General)
Uncontrolled Keywords:	Tumor cells, chloride channel blockers, cell volume, cell migration, transwell chamber.
Publisher:	Nature Publishing Group
ISSN:	1001-0602
Last Modified:	06 Jan 2022 02:22
URI:	https://orca.cardiff.ac.uk/id/eprint/62377

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