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Development of a reliable extraction and quantification method for glucosinolates in Moringa oleifera

Förster, Nadja, Ulrichs, Christian, Schreiner, Monika, Muller, Carsten Theodor and Mewis, Inga 2015. Development of a reliable extraction and quantification method for glucosinolates in Moringa oleifera. Food Chemistry 166 , pp. 456-464. 10.1016/j.foodchem.2014.06.043

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Abstract

Glucosinolates are the characteristic secondary metabolites of plants in the order Brassicales. To date the common DIN extraction ‘desulfo glucosinolates’ method remains the common procedure for determination and quantification of glucosinolates. However, the desulfation step in the extraction of glucosinolates from Moringa oleifera leaves resulted in complete conversion and degradation of the naturally occurring glucosinolates in this plant. Therefore, a method for extraction of intact Moringa glucosinolates was developed and no conversion and degradation of the different rhamnopyranosyloxy-benzyl glucosinolates was found. Buffered eluents (0.1 M ammonium acetate) were necessary to stabilize 4-α-rhamnopyranosyloxy-benzyl glucosinolate (Rhamno-Benzyl-GS) and acetyl-4-α-rhamnopyranosyloxy-benzyl glucosinolate isomers (Ac-Isomers-GS) during HPLC analysis. Due to the instability of intact Moringa glucosinolates at room temperature and during the purification process of single glucosinolates, influences of different storage (room temperature, frozen, thawing and refreezing) and buffer conditions on glucosinolate conversion were analysed. Conversion and degradations processes were especially determined for the Ac-Isomers-GS III.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 0308-8146
Date of Acceptance: 8 June 2014
Last Modified: 06 Mar 2019 16:08
URI: http://orca.cardiff.ac.uk/id/eprint/62564

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