New PCR assay detects rare tooth fungi in wood where traditional approaches fail

Parfitt, David, Hynes, Juliet, Rogers, Hilary Joan ORCID: https://orcid.org/0000-0003-3830-5857 and Boddy, Lynne ORCID: https://orcid.org/0000-0003-1845-6738 2005. New PCR assay detects rare tooth fungi in wood where traditional approaches fail. Mycological Research 109 (11) , pp. 1187-1194. 10.1017/S095375620500359X

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Abstract

Lu et al. (2002) described a method for identifying Hericium species by PCR, using the primers HT-U1 and HT-L1 which they specifically designed for this purpose. In our hands these primers do not appear to discriminate between tooth fungi and other wood decay species. Therefore PCR primers were designed that discriminated Creolophus cirrhatus from other species (HER2F/HER3R), and which discriminate Hericium alpestre, H. coralloides and H. erinaceus from other wood decay Ascomycota and Basidiomycota but not from each other (HER2F/HER2R). Using the HER2F/HER3R primers together with traditional isolation and direct incubation procedures, the location of C. cirrhatus in Turkey oak logs was mapped. The PCR approach often detected C. cirrhatus in locations where it was suspected to be, based on patterns of staining and decay, but where it was not revealed by isolation onto agar media, emphasising the value of adopting several approaches to unravel fungal community structure in wood.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > Q Science (General)
Publisher:	Elsevier
ISSN:	0953-7562
Date of Acceptance:	21 June 2005
Last Modified:	27 Oct 2022 08:32
URI:	https://orca.cardiff.ac.uk/id/eprint/62593

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