Analysis of microbial community functional diversity using sole-carbon-source utilisation profiles - a critique

Preston-Mafham, Juliet, Boddy, Lynne ORCID: https://orcid.org/0000-0003-1845-6738 and Randerson, Peter Frederick ORCID: https://orcid.org/0000-0002-2744-3122 2006. Analysis of microbial community functional diversity using sole-carbon-source utilisation profiles - a critique. FEMS Microbiology Ecology 42 (1) , pp. 1-14. 10.1111/j.1574-6941.2002.tb00990.x

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Abstract

Information on functional diversity (metabolic potential) is essential for understanding the role of microbial communities in different environments. Variations of the commercially available BIOLOG bacterial identification system plates are now widely used to assess functional diversity of microorganisms from environmental samples, based on utilisation patterns of a wide range (up to 95) of single carbon sources. There are many problems as well as benefits of using the approach, but the former are often disregarded. Here the basis of the approach is summarised, including type of plate to use, treatment of samples, replication, incubation conditions, monitoring of plates, and statistical analysis. The pros and cons of its use are critically assessed, inherent biases and limitations are pointed out and methodological difficulties are considered. Possible ways of overcoming some of the difficulties are suggested.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Uncontrolled Keywords:	Metabolic diversity; Substrate induced respiration (SIR); Microtitre plate; Community level physiological profile (CLPP); BIOLOG
Publisher:	Wiley-Blackwell
ISSN:	0168-6496
Date of Acceptance:	27 June 2002
Last Modified:	27 Oct 2022 08:38
URI:	https://orca.cardiff.ac.uk/id/eprint/62926

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