Insights into the gating mechanism of the ryanodine-modified human cardiac Ca2+- release channel (ryanodine receptor 2)

Mukherjee, Saptarshi, Thomas, Nia Lowri ORCID: https://orcid.org/0000-0001-8822-8576 and Williams, Alan John 2014. Insights into the gating mechanism of the ryanodine-modified human cardiac Ca2+- release channel (ryanodine receptor 2). Molecular Pharmacology 86 (3) , pp. 318-329. 10.1124/mol.114.093757

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Abstract

Ryanodine receptors (RyRs) are intracellular membrane channels playing key roles in many Ca2+ signaling pathways and, as such, are emerging novel therapeutic and insecticidal targets. RyRs are so named because they bind the plant alkaloid ryanodine with high affinity and although it is established that ryanodine produces profound changes in all aspects of function, our understanding of the mechanisms underlying altered gating is minimal. We address this issue using detailed single-channel gating analysis, mathematical modeling, and energetic evaluation of state transitions establishing that, with ryanodine bound, the RyR pore adopts an extremely stable open conformation. We demonstrate that stability of this state is influenced by interaction of divalent cations with both activating and inhibitory cytosolic sites and, in the absence of activating Ca2+, trans-membrane voltage. Comparison of the conformational stability of ryanodine- and Imperatoxin A-modified channels identifies significant differences in the mechanisms of action of these qualitatively similar ligands.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	American Society for Pharmacology and Experimental Therapeutics
ISSN:	1521-0111
Last Modified:	27 Oct 2022 08:39
URI:	https://orca.cardiff.ac.uk/id/eprint/62986

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