Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Generation of embryonic stem cells and transgenic mice expressing green fluorescence protein in midbrain dopaminergic neurons

Zhao, Suling, Maxwell, Sarah, Jimenez-Beristain, Antonio, Vives, Joaquim, Kuehner, Eva, Zhao, Jiexin, O'Brien, Carmel, de Felipe, Carmen, Semina, Elena and Li, Meng ORCID: https://orcid.org/0000-0002-4803-4643 2004. Generation of embryonic stem cells and transgenic mice expressing green fluorescence protein in midbrain dopaminergic neurons. European Journal of Neuroscience 19 (5) , pp. 1133-1140. 10.1111/j.1460-9568.2004.03206.x

Full text not available from this repository.

Abstract

We have generated embryonic stem (ES) cells and transgenic mice with green fluorescent protein (GFP) inserted into the Pitx3 locus via homologous recombination. In the central nervous system, Pitx3-directed GFP was visualized in dopaminergic (DA) neurons in the substantia nigra and ventral tegmental area. Live primary DA neurons can be isolated by fluorescence-activated cell sorting from these transgenic mouse embryos. In culture, Pitx3–GFP is coexpressed in a proportion of ES-derived DA neurons. Furthermore, ES cell-derived Pitx3–GFP expressing DA neurons responded to neurotrophic factors and were sensitive to DA-specific neurotoxin N-4-methyl-1, 2, 3, 6-tetrahydropyridine. We anticipate that the Pitx3–GFP ES cells could be used as a powerful model system for functional identification of molecules governing mDA neuron differentiation and for preclinical research including pharmaceutical drug screening and transplantation. The Pitx3 knock-in mice, on the other hand, could be used for purifying primary neurons for molecular studies associated with the midbrain-specific DA phenotype at a level not previously feasible. These mice would also provide a useful tool to study DA fate determination from embryo- or adult-derived neural stem cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Medicine
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: Dopaminergic neuron; green fluorescent protein (GFP); homologous recombination; homeobox transcription factor; Pitx3; stem cell.
Publisher: Wiley
ISSN: 0953-816X
Last Modified: 27 Oct 2022 08:41
URI: https://orca.cardiff.ac.uk/id/eprint/63104

Citation Data

Cited 143 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item