Increasing the efficiency of genomic searches for linkage in complex disorders by DNA pooling of affected sib-pairs

Craddock, Nicholas John ORCID: https://orcid.org/0000-0003-2171-0610, Daniels, J., Holmans, Peter Alan ORCID: https://orcid.org/0000-0003-0870-9412, Williams, Nigel Melville ORCID: https://orcid.org/0000-0003-1177-6931 and Owen, Michael John ORCID: https://orcid.org/0000-0003-4798-0862 1996. Increasing the efficiency of genomic searches for linkage in complex disorders by DNA pooling of affected sib-pairs. Molecular Psychiatry 1 (1) , pp. 59-64.

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Abstract

Detection of linkage using a systematic genome scan in nuclear families including an affected sibling pair is an important initial step on the path to cloning susceptibility genes for complex genetic disorders such as bipolar disorder and schizophrenia. We describe a novel method in which the pooled genotype of each affected sib-pair is determined and used in the screening stage of a two-stage genome scan. This method, which involves a single PCR reaction per sib-pair in the screening stage can reduce the required number of genotypings to less than 20% of those required in a conventional single stage procedure whilst maintaining a similar power and probability of type I error.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI) MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG) Neuroscience and Mental Health Research Institute (NMHRI)
Subjects:	R Medicine > R Medicine (General) R Medicine > RZ Other systems of medicine
Uncontrolled Keywords:	Substances Genetic Markers,DNA
Publisher:	Nature Publishing Group
ISSN:	1359-4184
Related URLs:	http://www.nature.com/mp/index.html
Last Modified:	27 Oct 2022 08:47
URI:	https://orca.cardiff.ac.uk/id/eprint/63390

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