Minisatellite DNA markers in the chicken genome. Isolation and characterization of minisatellite loci

Bruford, Michael William ORCID: https://orcid.org/0000-0001-6357-6080, Hanotte, O. and Burke, T. 1994. Minisatellite DNA markers in the chicken genome. Isolation and characterization of minisatellite loci. Animal Genetics 25 (6) , pp. 391-399. 10.1111/j.1365-2052.1994.tb00529.x

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Abstract

The isolation of chicken minisatellites is reported. Two charomid libraries were constructed from fractions of size-selected MboI-digested genomic DNA. A total of 5985 colonies were screened with the multilocus probes 33.6 and 33.15; 137 positives were obtained (2.35%). A total of 55 clones were tested against four unrelated chickens; 30 revealed variable single locus patterns. More detailed characterization was made of 15 probes, which were tested against four families comprising a total of 53 offspring. Of parent/offspring comparisons 51% were informative for segregation. No mutations were detected. Three minisatellites were linked on the same autosome, implying that these loci may occur in clusters, and one was Z chromosome-linked. Heterozygosity and allelic variability were measured in 67 individuals to several different strains and breeds. Mean heterozygosity ranged from 50 to 84%. Minisatellite loci appear to be highly variable in the chicken and should provide highly informative markers in intraspecific crosses in genome mapping studies.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Sustainable Places Research Institute (PLACES)
Publisher:	Wiley-Blackwell
ISSN:	1365-2052
Last Modified:	06 Jan 2024 02:57
URI:	https://orca.cardiff.ac.uk/id/eprint/65173

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